02604nas a2200229 4500008004100000245015700041210006900198260000800267300001000275490000600285520188500291653002902176653001802205653001302223653001502236653001402251653001602265653001302281100001902294700001902313856004202332 2016 eng d00aCapturing biodiversity: linking a cyanobacteria culture collection to the “scratchpads” virtual research environment enhances biodiversity knowledge0 aCapturing biodiversity linking a cyanobacteria culture collectio capr ae79650 v43 a
Abstract
Background
Currently, cyanobacterial diversity is examined using a polyphasic approach by assessing morphological and molecular data (Komárek 2015). However, the comparison of morphological and genetic data is sometimes hindered by the lack of cultures of several cyanobacterial morphospecies and inadequate morphological data of sequenced strains (Rajaniemi et al. 2005). Furthermore, in order to evaluate the phenotypic plasticity within defined taxa, the variability observed in cultures has to be compared to the range in natural variation (Komárek and Mareš 2012). Thus, new tools are needed to aggregate, link and process data in a meaningful way, in order to properly study and understand cyanodiversity.
New information
An online database on cyanobacteria has been created, namely the Cyanobacteria culture collection (CCC) ( http://cyanobacteria.myspecies.info/) using as case studies cyano- bacterial strains isolated from lakes of Greece, which are part of the AUTH culture collection (School of Biology, Aristotle University of Thessaloniki). The database hosts, for the first time, information and data such as morphology/morphometry, biogeography, phylogeny, microphotographs, distribution maps, toxicology and biochemical traits of the strains. All this data are structured managed, and presented online and are publicly accessible with a recently developed tool, namely “Scratchpads”, a taxon-centric virtual research environment allowing browsing the taxonomic classification and retrieving various kinds of relevant information for each taxon.
Keywords
cyanobacteria, database, Scratchpads, taxonomy, morphology, phylogeny, biodiversity informatics
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4500008004100000245003900041210003700080100001100117700002200128700001800150856005000168 1999 eng d00a. CYANOBACTERIA IN THE ENVIRONMENT0 aCYANOBACTERIA IN THE ENVIRONMENT1 aMur, R1 aSkulberg, Olav, M1 aUtkilen, Hans uhttps://cyanobacteria.myspecies.info/node/17502397nas a2200229 4500008004100000245009900041210006900140490000700209520170700216653002001923653002101943653002201964653002301986653001602009653001002025653001002035653002402045100001502069700001702084700001602101856005002117 2015 eng d00aIsolation and preliminary characterization of cyanobacteria strains from freshwaters of Greece0 aIsolation and preliminary characterization of cyanobacteria stra0 v103 aCyanobacterial harmful algal blooms (or CyanoHABs) represent one of the most conspicuous waterborne microbial hazards. The characterization of the bloom communities remains problematic because the cyanobacterial taxonomy of certain genera has not yet been resolved. In this study, 29 planktic and benthic cyanobacterial strains were isolated from freshwaters located in Greece. The strains were assigned to the genera Chroococcus, Microcystis, Synechococcus, Jaaginema, Limnothrix, Pseudanabaena, Anabaena, and Calothrix and screened for the production of the cyanotoxins microcystins (MCs), cylindrospermopsins (CYNs), and saxitoxins (STXs) using molecular (PCR amplification of seven genes implicated in cyanotoxin biosynthesis) and immunological (ELISA) methods. This study presents, for the first time, a cyanobacteria culture collection from Greece, thus providing missing study material for the understanding of bloom formation and cyanotoxin production in the Mediterranean and for the polyphasic characterization of important components of the phytoplankton. The combined use of molecular and immunochemical methods allowed the identification of MC producing strains, but further data are needed for CYN and STX-producing cyanobacteria. 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