02397nas a2200229 4500008004100000245009900041210006900140490000700209520170700216653002001923653002101943653002201964653002301986653001602009653001002025653001002035653002402045100001502069700001702084700001602101856005002117 2015 eng d00aIsolation and preliminary characterization of cyanobacteria strains from freshwaters of Greece0 aIsolation and preliminary characterization of cyanobacteria stra0 v103 a

Cyanobacterial harmful algal blooms (or CyanoHABs) represent one of the most conspicuous waterborne microbial hazards. The characterization of the bloom communities remains problematic because the cyanobacterial taxonomy of certain genera has not yet been resolved. In this study, 29 planktic and benthic cyanobacterial strains were isolated from freshwaters located in Greece. The strains were assigned to the genera Chroococcus, Microcystis, Synechococcus, JaaginemaLimnothrix, Pseudanabaena, Anabaena, and Calothrix and screened for the production of the cyanotoxins microcystins (MCs), cylindrospermopsins (CYNs), and saxitoxins (STXs) using molecular (PCR amplification of seven genes implicated in cyanotoxin biosynthesis) and immunological (ELISA) methods. This study presents, for the first time, a cyanobacteria culture collection from Greece, thus providing missing study material for the understanding of bloom formation and cyanotoxin production in the Mediterranean and for the polyphasic characterization of important components of the phytoplankton. The combined use of molecular and immunochemical methods allowed the identification of MC producing strains, but further data are needed for CYN and STX-producing cyanobacteria. The high percentage of MC-producing Microcystis strains in the urban Lakes Kastoria and Pamvotis, frequently used for agriculture irrigation, fishing and recreation, highlights the potential risk for human health.

10aAnabaena10aCalothrix10aLimnothrix10aMicrocystis10acyanotoxins10aELISA10alakes10amolecular detection1 aGkelis, S.1 aZaoutsos, N.1 aTussy, P.F. uhttps://cyanobacteria.myspecies.info/node/17000556nas a2200157 4500008004100000245012800041210006900169490000700238100001500245700001800260700001600278700002300294700001600317700001600333856004900349 2005 eng d00aLimnothrix redekei (Van Goor) Meffert (Cyanobacteria) Strains from Lake Kastoria, Greece Form a Separate Phylogenetic Group0 aLimnothrix redekei Van Goor Meffert Cyanobacteria Strains from L0 v491 aGkelis, S.1 aRajaniemi, P.1 aVardaka, E.1 aMoustaka-Gouni, M.1 aLanaras, T.1 aSivonen, K. uhttps://cyanobacteria.myspecies.info/node/56